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molecular imager ® versadoc™ mp imaging systems  (Bio-Rad)


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    Bio-Rad molecular imager ® versadoc™ mp imaging systems
    Molecular Imager ® Versadoc™ Mp Imaging Systems, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/molecular+imager+versadoc+mp+imaging+system/molecular+imager+versadoc+mp+4000+system/pmc11279630-121-21-28
    Average 90 stars, based on 1 article reviews
    molecular imager ® versadoc™ mp imaging systems - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Imaging:

    Article Title: Mechanism of regulation of tomato TRN1 gene expression in late infection with tomato leaf curl New Delhi virus (ToLCNDV).
    Article Snippet: Tomato leaf curl disease caused by geminiviruses is manifested by curling and puckering of leaves and thickening of veins, resembling developmental defects.. This is probably due to the long-term altered regulation of expression of development related gene(s).. Our results show that in the infected leaves the transcript level of TORNADO1 (SlTRN1), a gene important for cell expansion and vein formation, increased significantly.

    Article Title: In Vitro Cytotoxic Effect of Aqueous Extracts from Leaves and Rhizomes of the Seagrass Posidonia oceanica (L.) Delile on HepG2 Liver Cancer Cells: Focus on Autophagy and Apoptosis
    Article Snippet: The antibodies used were rabbit anti-p62/SQSTM1 (P0068, Sigma; working dilution 1:500), rabbit anti-Beclin-1 (SC-11427, Santa Cruz Biotechnology, Delaware, CA, USA; working dilution 1:500), rabbit anti-LC3 (L8918, Sigma; working dilution 1:1000), mouse anti-HSP60 (H3524, Sigma; working dilution 1:500), and rabbit anti-actin (Ab8227, Abcam; working dilution 1:1000). .. Subsequently, the membranes were washed exhaustively and incubated with peroxidase-conjugated anti-mouse or anti-rabbit secondary antibodies (W4021 and W4011, Promega Corporation, Madison, WI, USA) at room temperature for 1 h. Protein expression was visualized using a Molecular imager Versadoc MP imaging System (Bio-Rad, Hercules, CA, USA) and the Immun Star TM WesternC TM chemiluminescent kit (Bio-Rad). .. Bands’ quantitative analysis were carried out with Quantity One v.4.6.6 software (Bio-Rad) and referred to actin band intensity, used as a loading control.

    Article Title: Polycystic ovary syndrome: possible involvement of androgen-induced, chemerin-mediated ovarian recruitment of monocytes/macrophages
    Article Snippet: .. Signal intensity was assessed using Pierce ECL Western Blotting Substrate kit (Thermo Fisher) and Molecular Imager VersaDoc MP imaging system (Bio-Rad Laboratories, Inc). ..

    Article Title: The Synergy between Palmitate and TNF-α for CCL2 Production Is Dependent on the TRIF/IRF3 Pathway: Implications for Metabolic Inflammation
    Article Snippet: Resolved proteins were transferred to an Immun-Blot PVDF Membrane (Bio-Rad) by electroblotting, blocked with 5% nonfat milk in PBS for 1 h, and incubated overnight at 4°C with primary Abs (1:100 dilution; Cell Signaling Technology) against IRF3 and p-IRF3. .. Blots were washed four times with TBS and incubated for 2 h with HRP-conjugated secondary Ab (Promega, Madison, WI); immunoreactive bands were developed using an Amersham ECL Plus Western Blotting Detection System (GE Healthcare, Buckinghamshire, U.K.) and visualized using a Molecular Imager VersaDoc MP imaging system (Bio-Rad). ..

    Article Title: A Combined Transgenic Proteomic Analysis and Regulated Trafficking of Neuroligin-2
    Article Snippet: Membranes were blocked with 5% skim milk and 0.05% Tween 20 in Tris-buffered saline, and incubated with primary antibodies as follows: anti-GFP (1:1000; rabbit, A11122, Invitrogen), anti-neuroligin-2 (1:2000 (28)), anti-GABAAR 2 (1:1000; rabbit, NB300-151, Novus), anti-GABAAR 2 (1:1000; rabbit, Alomone Labs), anti-gephyrin (1:1000; mouse, SC25311, Santa Cruz), anti-synapsin1 (1:1000; rabbit, AB1543P, EMD Millipore), anti-PSD-95 family (recognizes PSD-95, PSD-93, SAP102, and SAP97; 1:1000; mouse IgG2a, 6G6-1C9, Thermo Scientific), and anti- -actin (1:2500; rabbit, Abcam). .. Following incubation in appropriate HRP-conjugated secondary antibodies, the signal was detected using the Immobilon Western Chemiluminescent HRP Substrate kit (WBKLS0100, Millipore) and imaged with the Bio-Rad Molecular Imager VersaDoc MP Imaging System (Bio-Rad). .. For silver staining, the PageSilver kit (K0681, Fermentas) was used.

    Article Title: A Combined Transgenic Proteomic Analysis and Regulated Trafficking of Neuroligin-2
    Article Snippet: Membranes were blocked with 5% skim milk and 0.05% Tween 20 in Tris-buffered saline, and incubated with primary antibodies as follows: anti-GFP (1:1000; rabbit, {"type":"entrez-nucleotide","attrs":{"text":"A11122","term_id":"490966"}} A11122 , Invitrogen), anti-neuroligin-2 (1:2000 ( 28 )), anti-GABA A Rγ2 (1:1000; rabbit, NB300-151, Novus), anti-GABA A Rα2 (1:1000; rabbit, Alomone Labs), anti-gephyrin (1:1000; mouse, SC25311, Santa Cruz), anti-synapsin1 (1:1000; rabbit, AB1543P, EMD Millipore), anti-PSD-95 family (recognizes PSD-95, PSD-93, SAP102, and SAP97; 1:1000; mouse IgG2a, 6G6-1C9, Thermo Scientific), and anti-β-actin (1:2500; rabbit, Abcam). .. Following incubation in appropriate HRP-conjugated secondary antibodies, the signal was detected using the Immobilon Western Chemiluminescent HRP Substrate kit (WBKLS0100, Millipore) and imaged with the Bio-Rad Molecular Imager VersaDoc MP Imaging System (Bio-Rad). .. For silver staining, the PageSilver kit (K0681, Fermentas) was used.

    Article Title: Variation of proteomic profile during lactation in Girgentana goat milk: a preliminary study
    Article Snippet: A representative 2D-map was obtained loading 30 lg of protein for SYPROVR Ruby (Life Technologies, Carlsbad, CA) staining and 500 lg for Coomassie Blue R-250 staining of the milk pooled sample. .. Gel images were acquired using the EttanDIGE imager scanner (GE Healthcare Life Sciences, Little Chalfont, UK) for SYPROVR Ruby staining and Molecular Imager VersaDoc MP Imaging System (Bio Rad, Hercules, CA) for Coomassie Brilliant Blue R-250 staining, according to manufacturer’s protocol. .. Protein pools preparation and labelling Protein pools (M1, M2, M3, F1 and F2) and not pooled samples (F1M1 and F2M1) were labelled using DIGE-specific dyes Cy2, Cy3 or Cy5 (GE Healthcare Life Sciences, Little Chalfont, UK) according to the manufacturer’s instructions.

    Article Title: TNF-α/Stearate Induced H3K9/18 Histone Acetylation Amplifies IL-6 Expression in 3T3-L1 Mouse Adipocytes
    Article Snippet: Blots were washed three times with TBST and incubated for 1h with HRP-conjugated secondary Ab (Promega, Madison, WI, USA). .. Immunoreactive bands were developed using an Amersham ECL Plus Western Blotting Detection System (GE Healthcare, Buckinghamshire, UK) and visualized using a Molecular Imager VersaDoc MP imaging system (Bio-Rad, Hercules, CA, USA). .. Data analysis was performed using GraphPad Prism software (La Jolla, CA, USA).

    Incubation:

    Article Title: In Vitro Cytotoxic Effect of Aqueous Extracts from Leaves and Rhizomes of the Seagrass Posidonia oceanica (L.) Delile on HepG2 Liver Cancer Cells: Focus on Autophagy and Apoptosis
    Article Snippet: The antibodies used were rabbit anti-p62/SQSTM1 (P0068, Sigma; working dilution 1:500), rabbit anti-Beclin-1 (SC-11427, Santa Cruz Biotechnology, Delaware, CA, USA; working dilution 1:500), rabbit anti-LC3 (L8918, Sigma; working dilution 1:1000), mouse anti-HSP60 (H3524, Sigma; working dilution 1:500), and rabbit anti-actin (Ab8227, Abcam; working dilution 1:1000). .. Subsequently, the membranes were washed exhaustively and incubated with peroxidase-conjugated anti-mouse or anti-rabbit secondary antibodies (W4021 and W4011, Promega Corporation, Madison, WI, USA) at room temperature for 1 h. Protein expression was visualized using a Molecular imager Versadoc MP imaging System (Bio-Rad, Hercules, CA, USA) and the Immun Star TM WesternC TM chemiluminescent kit (Bio-Rad). .. Bands’ quantitative analysis were carried out with Quantity One v.4.6.6 software (Bio-Rad) and referred to actin band intensity, used as a loading control.

    Article Title: The Synergy between Palmitate and TNF-α for CCL2 Production Is Dependent on the TRIF/IRF3 Pathway: Implications for Metabolic Inflammation
    Article Snippet: Resolved proteins were transferred to an Immun-Blot PVDF Membrane (Bio-Rad) by electroblotting, blocked with 5% nonfat milk in PBS for 1 h, and incubated overnight at 4°C with primary Abs (1:100 dilution; Cell Signaling Technology) against IRF3 and p-IRF3. .. Blots were washed four times with TBS and incubated for 2 h with HRP-conjugated secondary Ab (Promega, Madison, WI); immunoreactive bands were developed using an Amersham ECL Plus Western Blotting Detection System (GE Healthcare, Buckinghamshire, U.K.) and visualized using a Molecular Imager VersaDoc MP imaging system (Bio-Rad). ..

    Article Title: A Combined Transgenic Proteomic Analysis and Regulated Trafficking of Neuroligin-2
    Article Snippet: Membranes were blocked with 5% skim milk and 0.05% Tween 20 in Tris-buffered saline, and incubated with primary antibodies as follows: anti-GFP (1:1000; rabbit, A11122, Invitrogen), anti-neuroligin-2 (1:2000 (28)), anti-GABAAR 2 (1:1000; rabbit, NB300-151, Novus), anti-GABAAR 2 (1:1000; rabbit, Alomone Labs), anti-gephyrin (1:1000; mouse, SC25311, Santa Cruz), anti-synapsin1 (1:1000; rabbit, AB1543P, EMD Millipore), anti-PSD-95 family (recognizes PSD-95, PSD-93, SAP102, and SAP97; 1:1000; mouse IgG2a, 6G6-1C9, Thermo Scientific), and anti- -actin (1:2500; rabbit, Abcam). .. Following incubation in appropriate HRP-conjugated secondary antibodies, the signal was detected using the Immobilon Western Chemiluminescent HRP Substrate kit (WBKLS0100, Millipore) and imaged with the Bio-Rad Molecular Imager VersaDoc MP Imaging System (Bio-Rad). .. For silver staining, the PageSilver kit (K0681, Fermentas) was used.

    Article Title: A Combined Transgenic Proteomic Analysis and Regulated Trafficking of Neuroligin-2
    Article Snippet: Membranes were blocked with 5% skim milk and 0.05% Tween 20 in Tris-buffered saline, and incubated with primary antibodies as follows: anti-GFP (1:1000; rabbit, {"type":"entrez-nucleotide","attrs":{"text":"A11122","term_id":"490966"}} A11122 , Invitrogen), anti-neuroligin-2 (1:2000 ( 28 )), anti-GABA A Rγ2 (1:1000; rabbit, NB300-151, Novus), anti-GABA A Rα2 (1:1000; rabbit, Alomone Labs), anti-gephyrin (1:1000; mouse, SC25311, Santa Cruz), anti-synapsin1 (1:1000; rabbit, AB1543P, EMD Millipore), anti-PSD-95 family (recognizes PSD-95, PSD-93, SAP102, and SAP97; 1:1000; mouse IgG2a, 6G6-1C9, Thermo Scientific), and anti-β-actin (1:2500; rabbit, Abcam). .. Following incubation in appropriate HRP-conjugated secondary antibodies, the signal was detected using the Immobilon Western Chemiluminescent HRP Substrate kit (WBKLS0100, Millipore) and imaged with the Bio-Rad Molecular Imager VersaDoc MP Imaging System (Bio-Rad). .. For silver staining, the PageSilver kit (K0681, Fermentas) was used.

    Expressing:

    Article Title: In Vitro Cytotoxic Effect of Aqueous Extracts from Leaves and Rhizomes of the Seagrass Posidonia oceanica (L.) Delile on HepG2 Liver Cancer Cells: Focus on Autophagy and Apoptosis
    Article Snippet: The antibodies used were rabbit anti-p62/SQSTM1 (P0068, Sigma; working dilution 1:500), rabbit anti-Beclin-1 (SC-11427, Santa Cruz Biotechnology, Delaware, CA, USA; working dilution 1:500), rabbit anti-LC3 (L8918, Sigma; working dilution 1:1000), mouse anti-HSP60 (H3524, Sigma; working dilution 1:500), and rabbit anti-actin (Ab8227, Abcam; working dilution 1:1000). .. Subsequently, the membranes were washed exhaustively and incubated with peroxidase-conjugated anti-mouse or anti-rabbit secondary antibodies (W4021 and W4011, Promega Corporation, Madison, WI, USA) at room temperature for 1 h. Protein expression was visualized using a Molecular imager Versadoc MP imaging System (Bio-Rad, Hercules, CA, USA) and the Immun Star TM WesternC TM chemiluminescent kit (Bio-Rad). .. Bands’ quantitative analysis were carried out with Quantity One v.4.6.6 software (Bio-Rad) and referred to actin band intensity, used as a loading control.

    Western Blot:

    Article Title: Polycystic ovary syndrome: possible involvement of androgen-induced, chemerin-mediated ovarian recruitment of monocytes/macrophages
    Article Snippet: .. Signal intensity was assessed using Pierce ECL Western Blotting Substrate kit (Thermo Fisher) and Molecular Imager VersaDoc MP imaging system (Bio-Rad Laboratories, Inc). ..

    Article Title: The Synergy between Palmitate and TNF-α for CCL2 Production Is Dependent on the TRIF/IRF3 Pathway: Implications for Metabolic Inflammation
    Article Snippet: Resolved proteins were transferred to an Immun-Blot PVDF Membrane (Bio-Rad) by electroblotting, blocked with 5% nonfat milk in PBS for 1 h, and incubated overnight at 4°C with primary Abs (1:100 dilution; Cell Signaling Technology) against IRF3 and p-IRF3. .. Blots were washed four times with TBS and incubated for 2 h with HRP-conjugated secondary Ab (Promega, Madison, WI); immunoreactive bands were developed using an Amersham ECL Plus Western Blotting Detection System (GE Healthcare, Buckinghamshire, U.K.) and visualized using a Molecular Imager VersaDoc MP imaging system (Bio-Rad). ..

    Article Title: A Combined Transgenic Proteomic Analysis and Regulated Trafficking of Neuroligin-2
    Article Snippet: Membranes were blocked with 5% skim milk and 0.05% Tween 20 in Tris-buffered saline, and incubated with primary antibodies as follows: anti-GFP (1:1000; rabbit, A11122, Invitrogen), anti-neuroligin-2 (1:2000 (28)), anti-GABAAR 2 (1:1000; rabbit, NB300-151, Novus), anti-GABAAR 2 (1:1000; rabbit, Alomone Labs), anti-gephyrin (1:1000; mouse, SC25311, Santa Cruz), anti-synapsin1 (1:1000; rabbit, AB1543P, EMD Millipore), anti-PSD-95 family (recognizes PSD-95, PSD-93, SAP102, and SAP97; 1:1000; mouse IgG2a, 6G6-1C9, Thermo Scientific), and anti- -actin (1:2500; rabbit, Abcam). .. Following incubation in appropriate HRP-conjugated secondary antibodies, the signal was detected using the Immobilon Western Chemiluminescent HRP Substrate kit (WBKLS0100, Millipore) and imaged with the Bio-Rad Molecular Imager VersaDoc MP Imaging System (Bio-Rad). .. For silver staining, the PageSilver kit (K0681, Fermentas) was used.

    Article Title: A Combined Transgenic Proteomic Analysis and Regulated Trafficking of Neuroligin-2
    Article Snippet: Membranes were blocked with 5% skim milk and 0.05% Tween 20 in Tris-buffered saline, and incubated with primary antibodies as follows: anti-GFP (1:1000; rabbit, {"type":"entrez-nucleotide","attrs":{"text":"A11122","term_id":"490966"}} A11122 , Invitrogen), anti-neuroligin-2 (1:2000 ( 28 )), anti-GABA A Rγ2 (1:1000; rabbit, NB300-151, Novus), anti-GABA A Rα2 (1:1000; rabbit, Alomone Labs), anti-gephyrin (1:1000; mouse, SC25311, Santa Cruz), anti-synapsin1 (1:1000; rabbit, AB1543P, EMD Millipore), anti-PSD-95 family (recognizes PSD-95, PSD-93, SAP102, and SAP97; 1:1000; mouse IgG2a, 6G6-1C9, Thermo Scientific), and anti-β-actin (1:2500; rabbit, Abcam). .. Following incubation in appropriate HRP-conjugated secondary antibodies, the signal was detected using the Immobilon Western Chemiluminescent HRP Substrate kit (WBKLS0100, Millipore) and imaged with the Bio-Rad Molecular Imager VersaDoc MP Imaging System (Bio-Rad). .. For silver staining, the PageSilver kit (K0681, Fermentas) was used.

    Article Title: TNF-α/Stearate Induced H3K9/18 Histone Acetylation Amplifies IL-6 Expression in 3T3-L1 Mouse Adipocytes
    Article Snippet: Blots were washed three times with TBST and incubated for 1h with HRP-conjugated secondary Ab (Promega, Madison, WI, USA). .. Immunoreactive bands were developed using an Amersham ECL Plus Western Blotting Detection System (GE Healthcare, Buckinghamshire, UK) and visualized using a Molecular Imager VersaDoc MP imaging system (Bio-Rad, Hercules, CA, USA). .. Data analysis was performed using GraphPad Prism software (La Jolla, CA, USA).

    Staining:

    Article Title: Variation of proteomic profile during lactation in Girgentana goat milk: a preliminary study
    Article Snippet: A representative 2D-map was obtained loading 30 lg of protein for SYPROVR Ruby (Life Technologies, Carlsbad, CA) staining and 500 lg for Coomassie Blue R-250 staining of the milk pooled sample. .. Gel images were acquired using the EttanDIGE imager scanner (GE Healthcare Life Sciences, Little Chalfont, UK) for SYPROVR Ruby staining and Molecular Imager VersaDoc MP Imaging System (Bio Rad, Hercules, CA) for Coomassie Brilliant Blue R-250 staining, according to manufacturer’s protocol. .. Protein pools preparation and labelling Protein pools (M1, M2, M3, F1 and F2) and not pooled samples (F1M1 and F2M1) were labelled using DIGE-specific dyes Cy2, Cy3 or Cy5 (GE Healthcare Life Sciences, Little Chalfont, UK) according to the manufacturer’s instructions.



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